Technical Data
M9758-14
Myelin Proteolipid Protein, CT (MPLP)
Description:
This transmembrane proteolipid protein is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations.
Immunocytochemistry: 4% PFA fixed cells for 5-10 minutes at RT. Use 0.1% Triton in block only to allow antibody access to C-terminal epitope.
Immunohistochemistry (Frozen): Fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% Triton X-100 in the blocking step for permeabilization.
Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery.
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H410Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal, CGRGTKF of the Myelin proteolipid protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes the human myelin proteolipid protein (23-25kD). Crossreacts with DM20 (20kD). Species Crossreactivity: mammals
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
J. of Neuroscience. (1998) 18(15):5673-5681.