Technical Data
M9760-01E
Myeloperoxidase (MPO, pANCA) (PE)
Description:
Applications:
Suitable for use in Flow cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry (Neat): Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Detects myeloid cells following membrane permeabilization.
Optimal working dilutions to be determined by researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Controls:
I1904-79D IgG1 Murine Negative Control (PE)

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2762Affinity Purified
SizeStorageShippingSourceHost
100Tests4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~100 Tests/1ml
Immunogen:
Human myeloperoxiase.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilised powder from PBS, pH 7.4, 0.1% sodium azide, and 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml ddH2O.
Specificity:
Recognizes human myeloperoxidase (MPO), an important component of azurophilic granules in neutrophils.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Audrain, M.A.P. et al Anti-native and recombinant myeloperoxidasae monoclonals and human aytoantibodies. Clin. Exp. Immunol. 107: 127-134. (1997).