Technical Data
M9760-04N
Myeloperoxidase (MPO)
Description:
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains (12kD) and 2 heavy chains (60kD). This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. It is released in a degranulation process in response to bursts of oxygen consumption, which also forms H2O2. MPO and H2O2 form a complex that oxidases a large variety of substances some of which have microbicidal effects. The gene for MPO is translocated in patients with acute promyelocytic leukemia and is mutated in hereditary MPO deficiency.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Myeloperoxidase.
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human Myeloperoxidase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.