Technical Data
M9760-06
Myeloperoxidase (MPO, pANCA)
Description:
Myeloperoxidase is an important enzyme used by granulocytes during phagocytic lysis of engulfed foreign particles. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas. MPO is useful in differentiating between myeloid and lymphoid leukemias.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:100-1:800 for 20-30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Tonsil or spleen

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Purified human granulocytic MPO. Cellular Localization: Cytoplasmic
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human myeloperoxidase (MPO). Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chuang, S.S., et al., J. American Journal of Clinical Pathology, 107: 410 (1997). 2. Arber, D.A., et al., American Journal of Clinical Pathology, 106: 462-468 (1996).