Technical Data
M9760-10D
Myeloperoxidase (MPO, pANCA, 84kD Myeloperoxidase, 89kD Myeloperoxidase, Myeloperoxidase Heavy Chain, Myeloperoxidase Light Chain)
Description:
Myeloperoxidase is an important enzyme used by granulocytes during phagocytic lysis of foreign particles engulfed. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas. MPO is useful in differentiating between myeloid and lymphoid leukemias.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:1 Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6 for 10 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil or spleen

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
7ml-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the C-terminus of human Myeloperoxidase. Cellular Localization: Lysosomal.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, BSA, 0.1% sodium azide.
Specificity:
Recognizes human Myeloperoxidase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.