Technical Data
M9760-11
Myeloperoxidase (MPO, pANCA)
Description:
In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. Cells of monocytic derivation reveal variable reactivity and are typically weakly positive or nonreactive. Cells comprising the infiltrate of a spectrum of lymphoid malignancies, e.g., lymphoblastic lymphoma or leukemia, chronic lymphocytic leukemia, hairy cell leukemia, non-Hodgkin’s lymphomas of T-or B-cell type, and Hodgkin’s disease, are nonreactive, as are the non-neoplastic tissues present in these specimens, except for occasional cells of myeloid derivation. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas, or in the contiguous non-neoplastic tissues. MPO is useful in differentiating between myeloid and lymphoid leukemias.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:5000 detects multiple bands corresponding to
53kD and 15kD polypeptides and chain combination (68kD and 106kD)
Immunoprecipitation: 1:100
ELISA: 1:200,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
1ml-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Myeloperoxidase (human leukocytes)
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human Myeloperoxidase. Detects neutophilic granulocytes and monocytes in blood and precursors of granulocytes in bone marrow. May also detect myeloid leukemias of the bone marrow as well as other sites. Assay by immunoelectrophoresis resulted in a single precipitin arc against puri ed and partially puri ed Myeloperoxidase (human leukocytes).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.