Technical Data
M9850-15B
Myosin, Skeletal Muscle, heavy chain (MyHC)
Description:
Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Reported to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde.
Western Blot: 1:1000-1:5000. Detects Myosin Heavy Chain
(MyHC) in RIPA lysates from rabbit skeletal muscle cells. Rabbit skeletal cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M9850-15B (1:1000). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended Control:
RIPA lysates from C2C12 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG3J14Ascites
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Partially purified protein from adult human skeletal muscle myosin
Purity:
Ascites
Form
Supplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.035% sodium azide, 30% glycerol.
Specificity:
Recognizes human myosin heavy chain, Mr ~220kD. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products. Species Crossreactivity: mouse, rat, rabbit, zebrafish and Drosophila.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological Application Reference: 1. Huber, M.D., et al., Mol. Cell Biol. 29: 57185728 (2009). General Reference(s): 1. Cho, M., et al., J. Cell Sci. 107: 2361-2371 (1994). 2. Maggs, A.M., et al., J. Muscle Res. Cell Motil. 21: 101-113 (2000). 3. Blagden, C.S., et al., Genes Dev. 11: 2163-2175 (1997).