Technical Data
M9850-16X
Myosin, Smooth Muscle Heavy Chain (BSA & Azide Free)
Description:
SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:25 for 60 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Uterus

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F284Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Crude human uterus extract. Cellular Localization: Cytoplasmic. MW of Antigen: 205kD (MHC-1) and 200kD (MHC-2).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and sodium azide. See M9850-16.
Specificity:
Recognizes human MHC-1 (205kD) and MHC-2 (200kD) isoform, staining the intact myoepithelial cell layers present in benign and in situ malignant breast lesions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Frid, M.G., et al., Dev. Biol. 153: 185 (1992). 2. Glukhova, M.A., et al., J. Biol. Chem. 265(22): 13,042 (1990).