Technical Data
M9875
Myostatin Fusion Protein, Recombinant, Human (Lyophilized) (Growth Differentiation Factor 8, MSTN, gdf8)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12kD mature glycoprotein consisting of 109 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.

Human Myostatin is created as a recombinant protein with N-terminal fusion of HisTag with added 43 amino acid sequence as a spacer. The Human Myostatin-HisTag, produced in E. coli, is 16.7kD protein containing 109 amino acid residues of human Myostatin and 43 additional amino acid residues for a total of 152aa.

Human Myostatin is recombinant protein with N-terminal fusion of HisTag with added 43 amino acid sequence as a spacer: Met-Arg-Gly-Ser-His-His-His- His-His-His-Gly-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-Arg-Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys-Asp-Pro-Ser-Ser-Arg-Ser-Ala-Val-Arg-Ser-Arg-Arg.

AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSRSAVR SRRDFGLDCD EHSTESRCCR YPLTVDFEAF GWDWIIAPKR YKANYCSGEC EFVFLQKYPH THLVHQANPR GSAGPCCTPT KMSPINMLYF NGKEQIIYGK IPAMVVDRCG CS

Applications:
Suitable for use in Western Blot and ELISA.

Recommended Dilutions:
Optimal dilutions to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding acetate buffer. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
16.7kD
Source: Human, recombinant (E. coli).
Purity: 95% by SDS-PAGE. Endotoxin: 0.1ng per ug (1EU/ug).
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder from 0.05M acetate buffer, pH 4.5. Reconstitution: Reconstitute with 0.2ml of sterile 0.1M acetate buffer, pH 4. Allow the lyophilized pellet to dissolve completely. For conversion into higher pH value, intensive dilution by relevant buffer to 10ug/ml is recommended. In higher concentrations the solubility of this antigen is limited.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Artaza, J.N., et al., Endocrinology 146(8): 3547-3557 (2005). 2. Taylor, W.E., et al., Am. J. Physiol. Endocrinol. Metab. 280(2): E221-E228 (2001). 3. McPherron, A.C. & Lee, S.J., Proc. Natl. Acad. Sci. USA 94(23): 12,457-12,461 (1997). 4. Sharma, M., et al., J. Cell Physiol. 180(1): 1-9 (1999). 5. Gonzalez-Cadavid, N.F., et al., Proc. Natl. Acad. Sci. USA 95(25): 14,938-14,943 (1998). 6. Jiang, M.S., et al., Biochem Biophys. Res. Commun. 315(3): 525-531 (2004). 7. McPherron, A.C. & Lee, S.J., PNAS 98(16): 9306-9311 (2001). 8. Thies, R.S., et al., Growth Factors 18(4): 251-259 (2001). 9. Hill, J.J., et al., J. Biol. Chem. 277: 40,735-40,741 (2002). 10. Matsakas, A., et al., Exp. Physiol. 91(6): 983-994 (2006). 11. Ramazanov, Z., et al., Acta Physiol. Pharmacol. Bulg. 27(2-3): 101-106 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.