Technical Data
M9875-01C
Myostatin Fusion Protein, Recombinant, Human, Propeptide (Growth Differentiation Factor 8, MSTN, gdf8)
5ug
25ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Myostatin (GDF-8), a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.

Description: 28kD protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional amino acid residues. MG NENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN

Specificity:
The amino acid sequence of the recombinant human Myostatin Propeptide is 100% homologous to the amino acid sequence of the human Myostatin Propeptide without signal sequence.

Applications:
Western blotting, ELISA

Solubility:
Add 0.2 ml of d H2O and let the lyophilized pellet dissolve completely.

Stability:
Store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20C.

Molecular Weight:
28kD
Source: E. coli
Purity: 95% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder in 20mM Tris, 20mM sodium chloride, pH 8.0.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Work-induced changes in skeletal muscle IGF-1 and myostatin gene expression in uremia.
Kidney Int 2006 Jun 28;
2. Myostatin regulation of muscle development: Molecular basis, natural mutations, physiopathological aspects.
Exp Ce ll Res 2006 May 3;
3. Increased myostatin synthesis in rat gastrocnemius muscles under high-protein diet.
Int J Sport Nutr Exerc Metab 2006 Apr;16(2):153-65
4. Production of a monoclonal anti-myostatin antibody and the effects of in ovo administration of the antibody on posthatch broiler growth and muscle mass.
Poult Sci 2006 Jun;85(6):1062-71
5. Myostatin Regulates Cardiomyocyte Growth Through Modulation of Akt Signaling.
Circ Res 2006 Jun 8;
6. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep.
Nat Genet 2006 Jul;38(7):813-818

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.