Technical Data
M9875B
Myostatin Fusion Protein, Recombinant, Human (Lyophilized) (Growth Differentiation Factor 8, MSTN, gdf8)
2ug
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Myostatin is a TGF-beta family member that acts as an inhibitor of skeletal muscle growth. This muscle-specific cytokine interacts with Activin type I and type II receptors, and suppresses myoblast proliferation by arresting cell-cycle in the G1 phase. Suppression of myostatin activity facilitates muscle formation and may be useful in reducing and/or preventing adiposity and type-2 diabetes. Myostatin activity can be blocked by the Activin-binding protein Follistatin, and by the propeptide of Myostatin.

Recombinant Human myostatin is a 25kD protein consisting of two identical 109 amino acid polypeptides linked by a single disulfide bond.

Biological Activity:
Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17-25ng/ml.

AA Sequence:
DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with acetic acid. Add carrier protein for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin:
0.1ng/ug (1EU/ug).
Form: Supplied as a lyophilized powder. Reconstitute in sterile 10mM Acetic acid to 0.1-1.0mg/ml. Do not vortex. Add carrier protein for long term storage

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Gerard Y., Hober D., Petitjean S., Assicot M., Bohuon C., Mouton Y. & Wattre P.: High serum procalcitonin level in 4-year-old liver transplant recipient with a disseminated candidiasis. Infection 23., 310311 (1995) 2. Assicot M., Gendrel D., Carsin H., Raimond J., Guilbaud J. & Bohuon C.: High serum calcitonin concentrations in patients with sepsis and infection. Lancet 341, 515518 (1993)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.