Technical Data
M9875E
Myostatin, Recombinant, Human (E. coli) (Lyophilized) (Growth Differentiation Factor 8, MSTN, gdf8)
2ug
10ug
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass.Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. The myostatin pathway is conserved and regulates muscle mass in a number of animal species ranging from fish to humans. Inhibition of myostatin using a variety of therapeutic approaches can increase muscle mass in a number of animal models of human disease, including muscular dystrophy.

Endotoxin: 0.1EU/ug (LAL)

AA Sequence:
DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

Biological Activity: Recombinant Human Myostatin is fully biologically active when compared to standard. The ED50 as determined by the inhibition of the prolifiration of MPC-11 cells is < 20ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 20mM HCl, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
24.9kD
Source: Recombinant, human, E. coli
Purity: 95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. No Preservatives added.
Reconstitute with 20mM HCl to ~0.1mg/ml, which can be further diluted into other aqueous solutions. For long term storage, add a carrier protein (0.1% HSA or BSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.