Technical Data
M9925-09A
MYPT1, phosphorylated (Thr696) (Myosin Phosphatase 1)
Description:
Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1ug/ml detects MYPT1 phosphorylated at Thr969 on 10ug of Calyculin/Okadaic treated and untreated HEK293 cell lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Calyculin/Okadaic treated and untreated HEK293 cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to human phosphorylated Thr696 of MYPT1 (KLH).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM NaCl, 0.05% sodium azide.
Specificity:
Recognizes human MYPT1 phosphorylated at Thr696 at ~130kD. Species Crossreactivity: mouse. Species sequence homology: primate, canine, rat, Xenopus, equine, chicken, bovine, monkey, Danio.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.