-NAD Phosphate, Oxidized (Nicotinamide Adenine Dinucleotide) (b-NADPK)
|Biochemicals||Storage: -20°CShipping: Blue Ice|
Beta-nicotinamide adenine dinucleotide hydrate. Can be used as a cofactor in reactions with NAD-dependent histone deacetylase enzymes.
NAD is a coenzyme formed from the nucleotide, nicotinamide, adenosine monophosphate
and a phosphate group joining the first two components. NADP has the same structure with the addition of an extra phosphate group to AMP. NAD can be reduced to NADH during coupling with reactions which oxidize various organic substrates. For example, the reaction catalyzed by glyceraldehyde phosphate dehydrogenase during glycolysis. NADH then passes to the inside of mitochondria where it donates the electrons it is carrying to the electron transport chain. In this manner, NAD acts as an intermediate energy storage compound that indirectly generates ATP. Generally, NADP accepts electrons from catabolic reactions to form NADPH. An example is its coupling with the conversion of glucose-6-phosphate to ribose-5-phosphate in the pentose phosphate pathway. NADPH has a slightly different role to NADH in that it does not donate electrons to the electron transport chain. Instead, it tends to reduce intermediates in anabolic pathways e.g. fatty acid synthesis. NAD participates in many redox reactions in cells, including those in glycolysis and most of those in the citric acid cycle of cellular respiration.
Nicotinamide adenine dinucleotide (NAD) and its relative nicotinamide adenine dinucleotide phosphate (NADP) are two of the most important coenzymes in the cell. In cells, most oxidations are accomplished by the removal of hydrogen atoms. Both of these coenzymes play crucial roles in this. Each molecule of NAD+ (or NADP+) can acquire two electrons; that is, be reduced by two electrons. However, only one proton accompanies the reduction. The other proton produced as two hydrogen atoms are removed from the molecule being oxidized is liberated into the surrounding medium. For NAD, the reaction is thus:
NAD+ + 2H -> NADH + H+
UV Spectral Analysis:
Ratios at pH 7.5
A250/A260 (0.83 ± 0.03)
A280/A260 (0.21 ± 0.02)
e at 260 nm (18.0 ± 0.5) x 103
Ratios at pH 10 after reduction with G6PDH*
A340/A260 (0.43 ± 0.02)
e at 340 nm (6.2 ± 0.2) x 103
Potassium: 0% by flame photometyl
Water: 8% by Karl Fischer
Suitable for use as a calibrator or control serum.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: 95% determined by increase in absorbance at 340nm on enzymatic reduction with Glucose-6-phosphate dehydrogenase(yeast) (EC 188.8.131.52.) at pH 10.
Form: Supplied as lyophilized powder.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.