-NADP, Sodium Salt, Oxidized (Triphosphopyridine Nucleotide Sodium, TPN, Coenzyme 2)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
b-Nicotinamide adenine dinucleotide phosphate, sodium salt; Triphosphopyridine nucleotide sodium salt; TPN.
NAD is a coenzyme formed from the nucleotide, nicotinamide, adenosine monophosphate
and a phosphate group joining the first two components. NADP has the same structure with the addition of an extra phosphate group to AMP. NAD can be reduced to NADH during coupling with reactions which oxidize various organic substrates. For example, the reaction catalyzed by glyceraldehyde phosphate dehydrogenase during glycolysis. NADH then passes to the inside of mitochondria where it donates the electrons it is carrying to the electron transport chain. In this manner, NAD acts as an intermediate energy storage compound that indirectly generates ATP. Generally, NADP accepts electrons from catabolic reactions to form NADPH. An example is its coupling with the conversion of glucose-6-phosphate to ribose-5-phosphate in the pentose phosphate pathway. NADPH has a slightly different role to NADH in that it does not donate electrons to the electron transport chain. Instead, it tends to reduce intermediates in anabolic pathways e.g. fatty acid synthesis. NAD participates in many redox reactions in cells, including those in glycolysis and most of those in the citric acid cycle of cellular respiration.
Nicotinamide adenine dinucleotide (NAD) and its relative nicotinamide adenine dinucleotide phosphate (NADP) are two of the most important coenzymes in the cell. In cells, most oxidations are accomplished by the removal of hydrogen atoms. Both of these coenzymes play crucial roles in this. Each molecule of NAD+ (or NADP+) can acquire two electrons; that is, be reduced by two electrons. However, only one proton accompanies the reduction. The other proton produced as two hydrogen atoms are removed from the molecule being oxidized is liberated into the surrounding medium. For NAD, the reaction is thus:
NAD+ + 2H -> NADH + H+
743.41 (as anhydrous free acid)
White to off-white powder
Solubility (50mg/ml, H2O):
Clear, colorless to pale yellow
Water (KF): 8%
Solvent Content (GC): 6%
ICP Atomic Emission: As Reported
[enzymatic analysis with glucose-6-phosphate dehydrogenase at pH 7.5]
Sodium (AA): ~1-4%
1. at 260nm and pH7.5: (18.0 ± 0.8) x 103L.mole-1.cm-1
2. when reduced with G6PDH at 340nm and pH7.5:
(6.2 ± 0.3) x 103L.mole-1.cm-1
3. Ratios at pH 7.5: A250/A260 (0.83 ± 0.03)
A280/A260 (0.21 ± 0.02)
4. Ratio when reduced with G6PDH* at pH 7.5:
A340/A260 (0.43 ± 0.02)
Keep tightly stoppered in the dark below 5°C.
Moisture will reduce purity.
For prolonged storage, keep below -20°C.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.