Technical Data
NALP3 (NACHT, LRR and PYD Domains-containing Protein 3, Angiotensin/Vasopressin Receptor AII/AVP-like, Caterpiller Protein 1.1, CLR1.1, Cold Autoinflammatory Syndrome 1 Protein, Cryopyrin, PYRIN-containing APAF1-like Protein 1, NLRP3, C1orf7, CIAS1, PYPAF
NALP3 also known as CIAS1, PYPAF or Cryopyrin is a cytosolic ~120kD member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NALP3 activates caspases 1 and 5. Defects in NALP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Human NALP3 contains an N-terminal Pyrin domain aa1-93 followed by a Nacht region aa220-536, and seven LRRs aa740-991. Within the sequence used as an immunogen, human NALP3 shares 76% and 75% aa identity with mouse and rat NALP3, respectively. Isoforms of 979, 979, 922 and 719aa lack aa720776, aa836-892, both regions, or terminate at aa719.

Suitable for use in Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG112K139Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml after reconstitution
Recombinant corresponding to aa4-223 from human NALP3 expressed in E.coli.
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human NALP3. Does not react with recombinant human NALP1, 2, 5, rhNALP3 aa540-689, or recombinant mouse NALP3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.