Technical Data
N0020-01A
Natural Resistance Associated Macrophage Protein (Nramp2)
Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL technique
ELISA: 0.5-1ug/ml Use 1ug/ml control peptide/well
Immunohistochemistry: 5-20ug/ml. Use paraformaldehyde fixed sections.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
N0020-01: Natural Resistance Associated Macrophage Protein, Rat (Nramp2)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A 19 aa synthetic peptide sequence located at the predicted cytoplasmic loop #4 of rat NRAMP2 was synthesized, coupled to KLH. Species sequence homology: 100% mouse, 94% human, 73% zebra fish NRAMP2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes rat Natural Resistance Associated Macrophage Protein (Nramp2). This sequence is common in NRAMP2 with or without IRE isoforms. This antibody will not distinguish between these isoforms.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sharma N 2005 Biochem. J. (2005) 390 (437-446 A role for tumour necrosis factor a in human small bowel iron transport-WB, IHC. 2. Gunshin H et al (1997) Nature 388, 482; 3. Fleming MD et al (1998) PNAS 95, 1148; 4. Kishi F et al (1997) Mol Immunol. 6, 224; 5. Gruenshield S et al (1995) Genomics 25, 514; 6. Fleming MD et al (1997) Nature Genet. 16, 383; 7. Canonne-Hergaux F et al (1999) Blood 93, 4406 (review).