Technical Data
N0020-01B
Natural Resistance Associated Macrophage Protein (Nramp2)
Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 ECL.
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
N0020-02A Natural Resistance Associated Macrophage Protein, Rat, Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
Not determined.
Immunogen:
A 19 AA Peptide sequence located at the predicted cytoplasmic loop # 4 of rat NRAMP2 (1) (KLH)
Purity:
Serum.
Form
Supplied as a liquid, PBS, 40% glycerol.
Specificity:
Recognizes rat NRAMP2. Species cross-reactivity: Species sequence homology: mouse-100%, human-94%, zebra fish -73% This sequence is common in NRAMP2 with or without IRE isoforms. This antibody will not distinguish between these isoforms.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1). Gunshin H et al (1997) Nature 388, 482; Fleming MD et al (1998) PNAS 95, 1148; Kishi F et al (1997) Mol Immunol. 6, 224; Gruenshield S et al (1995) Genomics 25, 514; Fleming MD et al (1997) Nature Genet. 16, 383; Canonne-Hergaux F et al (1999) Blood 93, 4406 (review).