Technical Data
N0020-01C
Natural Resistance Associated Macrophage Protein (Nramp2)
Description:
Natural resistance to infection with unrelated intracellular parasite like Mycobacteria, Salmonella orLeishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed Nramp2, has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike Nramp1, Nramp2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. Nramp2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml using control peptide (N0020-01E) to coat plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Peptide corresponding 22aa located at the predicted cytoplasmic loop near the C-terminus of rat Nramp2, without IRE. Species Sequence Homology: human, mouse; 81%: monkey; 77%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes rat Natural Resistance Associated Macrophage Protein (Nramp2).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gunshin H et al (1997) Nature 388, 482. 2. Fleming MD et al (1998) PNAS 95, 1148. 3. Kishi F et al (1997) Mol Immunol. 6, 224. 4. Gruenshield S et al (1995) Genomics 25, 514. 5. Fleming MD et al (1997) Nature Genet. 16, 383. 6. Canonne-Hergaux F et al (1999) Blood 93, 4406 (review).