Technical Data
N0020-01F
Natural Resistance Associated Macrophage Protein (Nramp2)
Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide (Cat #N0020-01H) can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 5-20ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 17 AA Peptide sequence located at the predicted cytoplasmic loop near the C-terminus of human NRAMP2, with IRE (1). Coupled to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human Natural Resistance Associated Macrophage Protein (Nramp2). Species sequence homology: monkey 88%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Theurl I 2005 J. Hepatology, 43, 711-719 Pathways for the regulation of body iron homeostasis in response to experimental iron overload ihc.. 2. Yoon D 2006 J. Biol. Chem. 281: 25703 - 25711 HIF-1-deficiency results in dysregulated EPO signaling and iron homeostasis in mouse development IHC. 3. Khan ZA 2005 Free Radical Research, Nov2005, Vol. 39 Issue 11, p1203-IHC. Glucose-induced regulation of novel iron transporters in vascular endothelial cell dysfunction. 4. Sharma N 2005 Biochem. J. (2005) 390: 437-446 A role for tumour necrosis factor a in human small bowel iron transport IHC. 5. Gunshin H et al (1997) Nature 388, 482; 6. Fleming MD et al (1998) PNAS 95, 1148; 7. Kishi F et al (1997) Mol Immunol. 6, 224; 8. Gruenshield S et al (1995) Genomics 25, 514; 9. Fleming MD et al (1997) Nature Genet. 16, 383; 10. Canonne-Hergaux F et al (1999) Blood 93, 4406 (review).