Technical Data
N0020-01J
Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE)
Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 (slc11a2) gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'- UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. See catalog #N0020-02A: Natural Resistance Associated Macrophage Protein with IRE, Rat, Control Pepetide.
Western Blot: 1:1000-1:5000 (ECL).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide, 17aa located near the C-terminal, cytoplasmic domain of rat NRAMP2 conjugated to KLH. Sequence is 100% homologous with mouse Nramp2 with IRE and is absent in NRAMP2 without IRE. No significant sequence homology exists with NRAMP1.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes rat NRAMP2 with IRE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gunshin, H., et al., Nature 388: 482 (1997). 2. Fleming, M.D., et al., PNAS 95: 1148 (1998). 3. Kishi, F., et al., Mol. Immunol. 6: 224 (1997). 4. Gruenshield, S., et al., Genomics 25: 514 (1995). 5. Fleming, M.D., et al., Nature Genet. 16: 383 (1997). 6. Canonne-Hergaux, F., et al., Blood 93: 4406 (1999, review). 7. Roth, J.A., J. Neurosci. 20: 7595-7601 (2000). 8. Moos, T., J. of Neurochem. 88: 233-245 (2004).