		Technical Data
N0020-01J	Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE)

Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 (slc11a2) gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'- UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. See catalog #N0020-02A: Natural Resistance Associated Macrophage Protein with IRE, Rat, Control Pepetide.
Western Blot: 1:1000-1:5000 (ECL).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Rat	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide, 17aa located near the C-terminal, cytoplasmic domain of rat NRAMP2 conjugated to KLH. Sequence is 100% homologous with mouse Nramp2 with IRE and is absent in NRAMP2 without IRE. No significant sequence homology exists with NRAMP1.

Purity:

Serum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes rat NRAMP2 with IRE.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gunshin, H., et al., Nature 388: 482 (1997). 2. Fleming, M.D., et al., PNAS 95: 1148 (1998). 3. Kishi, F., et al., Mol. Immunol. 6: 224 (1997). 4. Gruenshield, S., et al., Genomics 25: 514 (1995). 5. Fleming, M.D., et al., Nature Genet. 16: 383 (1997). 6. Canonne-Hergaux, F., et al., Blood 93: 4406 (1999, review). 7. Roth, J.A., J. Neurosci. 20: 7595-7601 (2000). 8. Moos, T., J. of Neurochem. 88: 233-245 (2004).