Technical Data
N0020-01L
Natural Resistance Associated Macrophage Protein (Nramp1)
Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human NRAMP1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. NRAMP1 is expressed in the lysosomal compartment of monocytes and macrophages, lungs, and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. NRAMP1 (human 550 aa, chromosome 2q35; mouse 548 aa) protein is highly hydrophobic integral membrane glycoproteins composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
N0020-01M Natural Resistance Associated Macrophage Protein, Mouse Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
A 21 AA Peptide sequence located at the predicted cytoplasmic loop near the C-terminus of mouse NRAMP1 (KLH).
Purity:
Serum
Form
Supplied as a liquid in 40% glycerol.
Specificity:
The 21 AA mouse control peptide sequence is 59% conserved in red deer, bison, sheep, bovine, 57% in human NRAMP1. No significant sequence homology exist with NRAMP2. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1). Celier M et al (1994) J. Exp med. 180, 1741-1752; Kishi F et al (1995) Immunol. Lett. 47, 93-96; Kihsi F et al (1994) BBRC 204, 1074-1080; Blackwell JM et al (1995) Mol. Med. 1, 194-205; Liu J et al (1995) Am. J. Hum. Genet. 56, 845-853; Barton CH et al (1994) J Exp. Med. 179, 1683-1687; Govoni G et al (1995) Genomics 27, 9-19; Vidal SM et al (1993) Cell 469-485; Cannon-Hergaux F et al (2000) Proc. Assoc. Am. Phys. 111, 283-289 (review), Barton CH et al (1999) J. Leukoc. Biol. 66, 752-762 (review).