Technical Data
N0020-01P
Natural Resistance Associated Macrophage Protein (Nramp1)
Description:
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human NRAMP1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. NRAMP1 is expressed in the lysosomal compartment of monocytes and macrophages, lungs, and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. NRAMP1 (human 550aa, chromosome 2q35; mouse 548aa) protein is highly hydrophobic integral membrane glycoproteins composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N- and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml; Control peptide (Cat# N0020-01N) can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 17aa peptide sequence located at the predicted extracellular domain #4 of human Nramp1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes human Nramp1. Species Sequence Homology: red deer and sheep: 100%; bovine, water buffalo, bison: 93%; mouse: 76%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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