Technical Data
N0035-18
NaPi-4, NT, aa71-86 (Npt2a)
Description:
Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine. Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney. At least 4 groups of structurally and funcationally related proteins are in involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1, and NaPi-1 respectively in humans, mouse, and rabbit are expressed in the kidney and liver. Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear. Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opposum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal, and tubular Pi reabsorption. Deletion of Npt2 gene produces severe Pi wasting. Type II transporters are expressed in kidney, brain, lung, bone and small intestine.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml
Western Blot: 1-10ug/ml using Chemiluminescence technique.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceOpossumRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aaa71-86 within the cytoplasmic, N-terminus region of opossum NaPi-4, conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes opossum Napi-4. This sequence is not conserved in mouse, rat, human or other species Napi-4. No significant sequence homology is observed with other Pi-transporters (Type-I, III, or IV) transporters.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sorribas V et al (1994) JBC 269, 6615-6621. 2. Magnin S et al (1993) PNAS 90, 5979. 3. Collins JF et al (1996) FASEB J 8, 862;. 4. Hartman CM et al (1996) PNAS 93, 7409. 5. Murer H et al (2000) Physiol. Rev. 80, 1373. 6. Werner A et al (2001) Am. J. Physiol. Integ. Comp. Physiol. 280, R301