Technical Data
N0035-20
NaPi-1 (Npt1)
Description:
Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine. Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney. At least 4 groups of structurally and funcationally related proteins are in involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1, and NaPi-1 respectively in humans, mouse, and rabbit are expressed in the kidney and liver. Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear. Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opposum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal, and tubular Pi reabsorption. Deletion of Npt2 gene produces severe Pi wasting. Type II transporters are expressed in kidney, brain, lung, bone and small intestine. Type IIb, designated as NaPi-IIB or NaPi-2b in rat/mouse, and NaPi-3b in human, is closely related isoform of the NaPi-2 family. It is expressed in small intestine and lung. Type III NaPi transporters, originally described as a family of cell surface receptors for gibbon ape leukemia virus (GALV) and murine amphotropic retrovirus (A-MuLv), share very low (<20%) sequence homology with Type I and II proteins, and are found in most tissues. Human homolog are now desganted as PiT1 (also known as GALV receptors or GLVR1) and PiT2 (A-MuLV receptors or GLVR2 or Ram1). Pit1 and Pit2 are ~62% related and predicted to contain 10 TM domains that is in contrast with Type 1/2 transporters (6-8 TM). The N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.

Optimal dilutions to be determined by the researcher.

Control Peptide: N0035-22 NaPi-1, Mouse, Control Peptide (Npt1).

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Human NPT1 is 465/467aa (chromosome 6) and mouse and rat 465 aa proteins. A 15 AA synthetic peptide within the cytoplasmic, C-terminus of mouse Npt1 was synthesized, conjugated to KLH
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
The NPT11-P peptide is 93% conserved in 86% conserved in rat, 78% in human NPT1/Napi-4/NPT4 and 78% in rabbit Napi-1/NPT1. No significant sequence homology of NPT11-P is observed with other Pi-transporters (Type-II-IV) transporters. Antibody crossreactivity in various species is not established.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

(1) Chong SS et al (1995) Am. J. Phsiol. 268, F1038-F1045; Li H et al (1995) Cell. Mol. Biol. Res. 41, 451-460; Miyammoto K et al (1995) Biochem. J. 305, 81-85; Murer H et al (200) Physiological Rev. 80, 1373 (review); Werner A et al (2001) Am. J. Physiol. Integ. Comp. Physiol. 280, R301 (review).