		Technical Data
N0046-15A	NASH1 (SAM Domain-containing Protein SAMSN-1, Hematopoietic Adaptor Containing SH3 and SAM Domains 1, SAM Domain, SH3 Domain and Nuclear Localization Signals Protein 1, SH3-SAM Adaptor Protein, SAMSN1, HACS1)

Description:
Negative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.5-1.5ug/ml, observed in Jurkat Tcell lysate on ~48kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to IRLDTLSASLGRSC, from human SAMSN1, at N-terminal (NP_071419.2).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human SAMSN1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Zhu YX, Benn S, Li ZH, Wei E, Masih-Khan E, Trieu Y, Bali M, McGlade CJ, Claudio JO, Stewart AK. The SH3-SAM Adaptor HACS1 is Up-regulated in B Cell Activation Signaling Cascades. J Exp Med. 2004 Sep 20;200(6):737-47.