Technical Data
Brain Natriuretic Peptide (BNP)
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17aa ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (11 of the 17aa residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32aa peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.

Suitable for use in ELISA, RIA, Immunofluorescence and all staining techniques. Other applications not tested.

Recommended Dilutions:
Indirect Immunofluorescence: 1:1000 stains 4% buffered paraformaldehyde fixed rat brain sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceRabbit
Brain Natriuretic Peptide (BNP) covalently attached to a carrier protein.
Prepared by precipitation, chromatography and dialysis.
Supplied as a liquid in PBS. Dilute further in PBS, 10mg/ml BSA.
Recognizes Brain Natriuretic Peptide. Does not react with human or rat Atrial Natriuretic Peptide, human ACTH (aa1-39) or Vasoactive Intestinal Peptide. Species Crossreactivity: human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.