Technical Data
Brain Natriuretic Peptide (BNP)
Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesized as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.
BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.

Suitable for use in RIA, ELISA, Immunohistochemistry, Immunocytochemistry and Indirect Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:1000. Stains rat brain sections using 3% paraformaldehyde and indirect immunofluorescent staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic human brain natriuretic polypeptide covalently attached to a carrier protein.
Neat serum
Supplied as a lyophilized powder. Reconstitute with 100ul PBS, 10mg/ml BSA.
Recognizes human BNP (100%). Does not crossreact with Human Atrial Natriuretic Peptide, ACTH (human, 1-39), human Calcitonin, and Vasoactive Intestinal Peptide. Species crossreactivity: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Pieroni, M. et al., (2007) Eur Heart J 28: 1117-1127.