Technical Data
N0520-25
Brain Natriuretic Peptide (BNP)
Description:
Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesized as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.
BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.

Applications:
Suitable for use in RIA, ELISA, Immunohistochemistry, Immunocytochemistry and Indirect Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:1000. Stains rat brain sections using 3% paraformaldehyde and indirect immunofluorescent staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic human brain natriuretic polypeptide covalently attached to a carrier protein.
Purity:
Neat serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul PBS, 10mg/ml BSA.
Specificity:
Recognizes human BNP (100%). Does not crossreact with Human Atrial Natriuretic Peptide, ACTH (human, 1-39), human Calcitonin, and Vasoactive Intestinal Peptide. Species crossreactivity: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Pieroni, M. et al., (2007) Eur Heart J 28: 1117-1127.