Technical Data
N0520-25A
Brain Natriuretic Peptide (BNP)
Description:
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 aa ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 aa residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 aa peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.

Applications:
Suitable for use in RIA, ELISA, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Brain Natriuretic Polypeptide covalently attached to bovine thyroglobulin.
Purity:
Serum
Form
Supplied as a lyophilized powder. No preservative added. Reconstitute with 0.1ml PBS, 10mg/ml BSA.
Specificity:
Recognizes human Brain Natriuretic Peptide (BNP). Species Crossreactivity: rat. Cross-reactivity: Brain Natriuretic Peptide: 100%; Human Atrial Natriuretic Peptide: 0%; ACTH (human, 1-39): 0%; Calcitonin (human): 0%; Vasoactive Intestinal Peptide: 0%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.