Technical Data
N0536-05
NBC1 (HCO3 Cotransporters 1, pNBC)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (Chemiluminescence technique)
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide (N0536-06q) /well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A 20-aa peptide sequence within the cytoplasmic, N-terminus of rat pNBC1 was used to generate anti-NBC1. The peptide was coupled with KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat NBC1. This sequence is 100% conserved in mouse, rat, human and rabbit pNBC1/PSLC4A5. This region is unique to the pancreatic isoforms and it is not found in kidney isoform, kNBC/PSLC4A4. No significant sequence homology of was found with NBC2 or NBC3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References Romero MF et al (1998) Am. J. Physiol. 274, F425-F432 Burnham CE et al (1998) gene accession # AF027362; Burnham CE et al (1997) J. Biol. Chem. 272, 19111-19114; Abuladze N et al (1998) J. Biol. Chem. 273; 17689-17695; Giffard RG et al (2000) J. Neurochem. In press; Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723 (review)