Technical Data
N0536-05A
NBC1 (Na, HCO3 Cotransporter 1, pNBC)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
NBC1 (human, rat, mouse 1035 aa, also called NBC-1A/1B, hkNBC, rkNBC, pNBC, hhNBC, SLC4A4) was initially cloned from human Kidney. NBC1 is 30-35% identical with AEs. It is strongly expressed in the kidney and pancreas. The rat kidney NBC1 (rkNBC1; 1035 aa) is 86% identical to hkNBC1. Pancreatic NBC (pNBC/hhNBC/SLC4A5, human 1079 aa) has a unique n-terminal 85 aa sequence replacing the 41-aa in kNBC. pNBC is also more widely expressed (pancreas, thyroid, heart, and brain).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10K-1:100K using 50-100ng of control peptide/well.
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
1mg/ml
Immunogen:
A 20-aa peptide sequence within the cytoplasmic, N-terminus of human NBC1 (1) was used to generate anti-NBC1. The peptide was coupled with KLH (carrier protein)
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Human sequence is 100% conserved in mouse, rat, human and rabbit NBC1/PSLC4A4/kNBC and 75% in frog NBC1. This region is also shared 100% with pNBC/ PSLC4A5. Therefore, antibodies will recognize both the kidney and pancreatic/heart isoforms. No significant sequence homology of was found with NBC2 or NBC3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Romero MF et al (1998) Am. J. Physiol. 274, F425-F432 Burnham CE et al (1998) gene accession # AF027362; Burnham CE et al (1997) J. Biol. Chem. 272, 19111-19114; Abuladze N et al (1998) J. Biol. Chem. 273; 17689-17695; Giffard RG et al (2000) J. Neurochem. In press; Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723 (review)