Technical Data
N0537-05
NBC2 (HCO3 Cotransporters 2)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1- SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
BC2 (PSLC4A6; human 1018aa), initially isolated from retina, is only 53% identical to NBC1. It is widely expressed in retinal, testis, spleen, colon, small intestine, ovary, thymus, prostate, skeletal muscle, heat, kidney, stomach and bone marrow. It appears to be absent in pancreas and liver.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using Chemiluminescence technique.
ELISA: 1:5000-1:50,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
N0537-15 NBC2, Human Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 20aa peptide sequence within the cytoplasmic, C-terminus of human NBC2 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.
Specificity:
Recognizes human NBC2. No significant sequence homology was found with NBC1 or NBC3. Species sequence homology: rat-100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishibashi, K., et al., BBRC 246: 535-538 (1998). 2. Solemani, M., et al., Kidney Intl. 57: 371-384 (2000) (review). 3. Romero, M.F., et al., Ann. Rev. Physiol. 61: 699-723 (1999) (review).