Technical Data
N0538-06
NBC3 (HCO3 Cotransporters 3)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, thebe tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000. Using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
N0538-10: NBC3, Human (HCO3 Cotransporters 3)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A 19-aa peptide sequence within the C-terminus, cytoplasmic domain of human NBC3.
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes human NBC3 (HCO3 Cotransporters 3). Species sequence homology: chimp,100%,; rat, 83%; Xenopus, 68%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pushkin A et al (1999) J. Biol. Chem. 274, 16569-16575; 2. Choi I et al (1999) gene Accession # AF070475; 3. Amlal H et al (1999) Am. J. Physiol. 276, F903-F913; 4. Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); 5. Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723