Technical Data
N0538-08
NBC3 (HCO3 Cotransporters 3)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). NBC3 (SLC4A); mouse (1089 aa, human 1044 aa) has 3 three transcripts, which are expressed in a tissue specific manner (9 kb in brain, placenta, lung, liver, muscle, kidney, pancreas, stomach, thyroid, and adrenal etc). NBC3 has two variants. NBC3 is 56% identical with NBC1 and 76% with NBC2.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
N0538-10: NBC3, Human (HCO3 Cotransporters 3)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 19-aa peptide sequence within the cytoplasmic, C-terminus of human NBC3. (KLH).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human NBC3 (HCO3 Cotransporters 3). Species sequence homology: Chimp, 100%; rat, 83%; and Xenopus 68%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pushkin A et al (1999) J. Biol. Chem. 274, 16569-16575; Choi I et al (1999) gene Accession # AF070475; 2. Amlal H et al (1999) Am. J. Physiol. 276, F903-F913; 3. Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); 4. Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723 (review).