Technical Data
N0538-15
NBC3 (HCO3 Cotransporters 3)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting : 1-10ug/ml for affinity pure using Chemiluminescence technique.
ELISA: (1:10K-1:100K using 50-100ng of control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
A 16-aa peptide sequence within the cytoplasmic, N-terminus of human NBC3 (1) was used to generate anti-NBC1
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
Human sequence is 100% conserved in mouse NBC3. No significant sequence homology of was found with NBC1 or NBC2. Antibody crossreactivity in various species is not established. We recommend the use of control peptide in antibody blocking experiments to establish antibody specificity. Control peptides, because of their small size (< 2kD), are not suitable for Western
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References Pushkin A et al (1999) J. Biol. Chem. 274, 16569-16575; Choi I et al (1999) gene Accession # AF070475; Amlal H et al (1999) Am. J. Physiol. 276, F903-F913; Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723 (review)