Technical Data
N0568
NdeI
4000U
Molecular Biology Storage: -20CShipping: Blue Ice
5'-C A^T A T G-3'
3'-G T A T^A C-5'

Buffers:
R1625-Restriction Enzyme Buffer A (double digests): 1X Buffer composition-Supplied as a liquid containing 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37C.

R1625-03-Restriction Enzyme Buffer D (for 100% digestion): 1X Buffer composition-Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37C.

Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with NdeI.

Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with NdeI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.

Stability during Prolonged Incubation: A minimum of 0.3 units of Ndel is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Thermal Inactivation: Ndel is inactivated by incubation at 65C for 20min.

Digestion of Agarose-embedded DNA: A minimum of 5 units of Ndel is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: Csp6I, FspBI, Tru1I, VspI

Number of Recognition Sites in DNA:
Lambda: 7
PhiX174: 0
M13mp18/19: 3
pBR322: 1
pUC18/19: 1
pUC57: 1
pTZ19R/U: 0

Unit Definition:
One unit is defined as the amount of Ndel required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.

Dilution Buffer:
10mM Tris-HCl, pH 7.4 at RT, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

Enzyme Activity in Buffer A (R1625)/Buffer D (R1625-03):
R1625-0-20%
R1625 (2X)-50-100%
R1625-03-100%
Source: Neisseria denitrificans
Concentration: ~10u/ul
Form: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.