		Technical Data
N0570-12F	NDC80 Homolog, Kinetochore Complex Component (Highly Expressed in Cancer, HEC, HEC1, HsHec1, hsNDC80, Kinetochore Associated 2, KNTC2, Kinetochore Protein Hec1, Kinetochore Protein NDC80 Homolog, NDC80, Retinoblastoma Associated Protein HEC, TID3, NDC80 H

Description:
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1,000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	11C462	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Recombinant protein corresponding to aa545-642 of human KNTC2.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human KNTC2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.