Technical Data
N0570-12F
NDC80 Homolog, Kinetochore Complex Component (Highly Expressed in Cancer, HEC, HEC1, HsHec1, hsNDC80, Kinetochore Associated 2, KNTC2, Kinetochore Protein Hec1, Kinetochore Protein NDC80 Homolog, NDC80, Retinoblastoma Associated Protein HEC, TID3, NDC80 H
Description:
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1,000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111C462Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant protein corresponding to aa545-642 of human KNTC2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human KNTC2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.