Technical Data
N1193-10M
Netrin G1 Ligand Precursor (NGL-1, LRRC4C, leucine rich repeat containing 4C KIAA1580, UNQ292, PRO331) (Biotin)
Description:
The Netrin-G1 ligand (NGL-1) promotes the outgrowth of thalamocortical axons. Human netrin G1 ligand is a transmembrane protein containing leucine-rich repeat (LRR) and immunoglobulin (Ig) domains that specifically interacts with netrin-G1 through its LRR region. Postsynaptic netrin-G ligand (NGL) CAMs associate with netrin-G CAMs in an isoform-specific manner and, through their cytosolic tail, with the abundant postsynaptic scaffold postsynaptic density-95 (PSD-95).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Westerm Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human NGL-1. The detection limit for rhNGL-1 is approximately 2ng/lane under non-reducing and reducing
conditions. Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanSheep
Concentration:
~50ug/ml
Immunogen:
Purified, NS0-derived, recombinant human NGL-1 extracellular domain (rhNGL-1)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS, 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base,150mM NaCl), 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
Specificity:
Recognizes human NGL-1, extracellular domain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.