Technical Data
N1193-15
Netrin G2a
Description:
Mouse Netrin G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin G2a is approximately 485aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59aa and 34aa between EGF-like motifs 1 and 2 generate Netrin G2b and Netrin G2c, respectively. Mature mouse Netrin G2a shares 96% and 99% amino acid identity with human Netrin G2 and the equivalent region in rat Netrin G2b, respectively.

Applications:
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Netrin G2a.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7H116Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa18-504 from mouse Netrin G2a expressed in NSO cells (NP_598007).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes mouse Netrin G2a in direct ELISAs and Western Blots. Does not crossreact with recombinant mouse Netrin G1a, Netrin 1 or Netrin 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.