Technical Data
Netrin G2a
Mouse Netrin G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin G2a is approximately 485aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59aa and 34aa between EGF-like motifs 1 and 2 generate Netrin G2b and Netrin G2c, respectively. Mature mouse Netrin G2a shares 96% and 99% amino acid identity with human Netrin G2 and the equivalent region in rat Netrin G2b, respectively.

Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections.
Optimal dilutions to be determined by the researcher.

Mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Netrin G2a.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a7H116Affinity Purified
100ug-20CBlue IceMouseRat
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding to aa18-504 from mouse Netrin G2a expressed in NSO cells (NP_598007).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes mouse Netrin G2a in direct ELISAs and Western Blots. Does not crossreact with recombinant mouse Netrin G1a, Netrin 1 or Netrin 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.