Technical Data
N1193-16
Netrin 1 (Netrin 1, Ntn1, NTN1L, Unc6)
Description:
Murine netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various non-neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of commissural neurons from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:500
Immunohistochemistry: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanChicken
Concentration:
~0.1mg/ml
Immunogen:
Peptide corresponded to a region of the Netrin 1 gene product shared between the mouse (O09118, NCBI) and human (O95631, NCBI) gene products.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human Netrin-1. Species crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.