Technical Data
N1193-16
Netrin 1 (Netrin 1, Ntn1, NTN1L, Unc6)
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Technical Data |
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N1193-16 |
Netrin 1 (Netrin 1, Ntn1, NTN1L, Unc6) |
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Description: Murine netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various non-neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of commissural neurons from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:500 Immunohistochemistry: 1:100-1:200 Immunocytochemistry: 1:100-1:200 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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