Technical Data
N1193-16B
Netrin 1 (Netrin 1, Ntn1, NTN1L, Unc6)
Description:
Semaphorins, neuropilins and netrins are among a number of molecules and their receptors that regulate the developing nervous system to guide the development of neural circuits.1 Although first identified as axon guidance cues,2,3 it is now apparent that many of these same factors are not limited to the guidance of growing axons, but have roles in a range of processes from the guidance of cell migration to the regulation of the immune response, angiogenesis, lung branching morphogenesis, nervous system regeneration, and cancer.4-9
The semaphorins make up the largest family of axon guidance cues. They are characterized by the presence of an approximately 500 amino acid N-terminal semaphorin (Sema) domain. Semaphorins function mainly as chemorepellents that direct axons away from tissues.3 Semaphorin 3A (Sema3A) has been shown to be repellent to cortical axons and to inhibit axon branching.10 The transmembrane protein semaphorin 6A has been shown to repel embryonic sympathetic axons.11 The actions of the various semaphorins are not always similar. Semaphorin 3A has been found to inhibit tumor development whereas semaphorin 6A may contribute to tumor progression.9

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9A20Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Purified, recombinant protein corresponding to mouse Netrin-1, expressed in NS0 cells
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 5% trehalose.
Specificity:
Recognizes mouse Netrin 1. Species crossreactivity: recombinant chicken Netrin-1. Does not crossreact with recombiant chicken Netrin-2, recombinant human Netrin-4, recombinant mouse Netrin-4 or recombinant mouse Netrin-G1a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brian Reeves W, Kwon O, Ramesh G. Netrin-1 kidney injury. II. Netrin-1 is an early biomarker of acute kidney injury. Am J Physiol Renal Physiol;294(4):F731-8. 1-Apr-08. [PMID: 18234954]