Technical Data
N1193-16C
Netrin 1 (NTN1, NTN1L)
Description:
Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml. ~70kD band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 67.7kD according to Human NP_004813.1 and 67.8kD according to Rat NP_446183.1).
Optimal dilutions to be determined by the researcher.

Peptide Blocking:
N1193-16C-P: Netrin 1, corresponding peptide; Peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration is 0.5mg/ml. Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20 minutes. Then incubate and develop the two blots in parallel.

Recommended Positive Control:
Human Brain (Cerebellum), Mouse and Rat Brain lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide KFQQREKKGKCKKA from the C-Terminus of human Netrin 1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes rat Netrin 1 at ~70kD. Species Crossreactivity: human, mouse. Species sequence homology: canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lin L, Rao Y, Isacson O. Netrin-1 and slit-2 regulate and direct neurite growth of ventral midbrain dopaminergic neurons. Mol Cell Neurosci. 2005 Mar;28(3):547-55.