Technical Data
N1193-16D
Netrin 1 (NTN1)
Description:
Murine netrin-1 precursor is a 67.768 Kd protein (604aa) expressed by subpopulations of cells within the developing CNS, as well as in various non- neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of commissural neurons from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.

Applications:
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseChicken
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to Netrin-1 (KLH). Species sequence homology: human (O_095631, NCBI) and mouse (O_09118, NCBI)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes Netrin-1. Species crossreactivity: mouse. Species sequence homology: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.