		Technical Data
N1260-01	Neuroligin 1 (NLGN1)

Description:
Neuroligin 1 is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. In addition, the neuroligin/beta-neurexin junction may be involved in the excitatory/inhibitory specification of CNS neuron synapses. A role for Neuroligin 1 is also suggested in autism.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100,000 using 50-100ng of N1260-01A control peptide/well
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to 17aa of the C-terminus of human NLGN1 (KLH). Species sequence homology: mouse and rat.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Specificity:

Recognizes human Neuroligin 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lezcano, N Science 287:1660-1664, 2000.