Technical Data
N1260-03A
NEUROLIGIN 3 (NLGN3, ASPGX1, AUTSX1, Gliotactin Homolog, HNL3, KIAA1480, Neuroligin-3, NL3)
Description:
This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but their full length sequences have not been determined.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:16,000
Western Blot: 0.05-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain (Amygdala) lysates

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See N1260-03A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-RKDKRRQEPLRQPS, from the internal region of human NLGN3.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human NLGN3 at ~90kD. Species sequence homology: mouse, rat, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Tabuchi K, Blundell J, Etherton MR, Hammer RE, Liu X, Powell CM, Sudhof TC. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science. 2007 Oct 5;318(5847):71-6. Epub 2007 Sep 6.