Technical Data
N1260-03C
Neuroligin 3 (NLGN3)
Description:
Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects Neuroligin-3 on 10ug of rat brain membrane tissue lysate.
Immunohistochemistry (paraffin): 1:400 detects Neuroligin-3 in rat brain (putamen area) and rat cerebellum tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain membrane tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k11C249Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to rat Neuroligin-3 at the cytoplasmic domain.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes rat Neuroligin-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.