Technical Data
N2021-50
NHE7 (Na, H Exchanger 7)
Description:
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE7 (human 725aa, chromosome Xp11.4) is ubiquitously expressed, and predominantly localizes to the trans-golgi network. NHE7 mediates the influx of Na+ or K+ in exchange for H+. It is ~70% related to NHE6 but relatively less (~25%) homolohgous with other NHEs.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 1:100,000 using 50-100ng control peptide/well.
Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissues.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
N2021-75: NHE7, Human (Na, H Exchanger 7)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstittion)
Immunogen:
Synthetic peptide corresponding to 17aa from human NHE7 at N-terminal, conjugated to KLH. Species sequence homology: chimp;100%, mouse and rat; 94%. Sequence homology: NHE6; 70%, but relatively less with other NHEs; 25% .
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.5, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human NHE7 (Na, H Exchanger 7).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smith DR. 2004, Experimental Cell Research, Vol 298, Issue 2, 15 Aug 2004, Pgs 521-534. 2. Numata M et al (2001) JBC 276, 17386-17394; 3. Chris Yun CH et al (1995) Am J Physiol. 269, G1-G11 (Review); 4. Josette N and Pouyssegur J (1995) Am J Physiol. 268, C283-C296 (review).