Technical Data
N2025-02E
Nherf1 (Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding Phosphoprotein 50, EBP50, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium-hydrogen Exchanger Regulatory Factor 1, Solute Carrier Family 9 Isoform A3 Regulat
Description:
NHE3 regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein that interacts with NHE3 via its PDZ domains. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa338 from rat NHERF1.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 5mM EDTA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes rat NHERF1/EBP50. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.