		Technical Data
N2025-02E	Nherf1 (Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding Phosphoprotein 50, EBP50, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium-hydrogen Exchanger Regulatory Factor 1, Solute Carrier Family 9 Isoform A3 Regulat

Description:
NHE3 regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein that interacts with NHE3 via its PDZ domains. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Rat	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa338 from rat NHERF1.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 5mM EDTA, 0.01% thimerosal, 30% glycerol.

Specificity:

Recognizes rat NHERF1/EBP50. Species Crossreactivity: human and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.