Technical Data
Nephrin (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)
Nephrin was first described as a kidney podocyte marker protein with a MW of 135kD calculated from amino acid sequence data and an apparent Mr 185kD after SDS-PAGE. Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.

Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:50 Microwave antigen retreval required.
Immunofluorescence: Frozen tissue
Western Blot: 1:500; 1 hour at RT or overnight at 4C
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceGuinea pig
Not Determined
Synthetic peptide corresponding to the intracellular domain, aa1243-1256 of Nephrin.
Supplied as a liquid, 0.09% sodium azide.
Recognizes nephrin. Positively stains podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas. Reacts with PCL (podocyte cell line) and M-1 (cortical collecting duct cells). Species Crossreactivity: human and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kestila, M., et al., Mol. Cell. 4: 575-582 (1998). 2. Ruotsalainen, V., et al., Proc. Natl. Acad. Sci. USA. 96: 7962-7967 (1999). 3. Holthofer, H., et al., Am. J. Pathol. 155: 1681-1687 (1999). 4. Putaala, H., et al., Hum. Mol. Genet. 10: 1-8 (2001). 5. Huh, W., et al., Nephrol. Dial. Transplant 17: 478-484 (2002). 6. Aaltonen, P., et al., Lab. Invest. 81: 1185-1190 (2001). 7. Bussolati, B., et al., J. Am. Soc. Nephrol. 16: 1936-1947 (2005).