Technical Data
N2028-50
Nephrin (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)
Description:
Nephrin was first described as a kidney podocyte marker protein with a MW of 135kD calculated from amino acid sequence data and an apparent Mr 185kD after SDS-PAGE. Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:50 Microwave antigen retreval required.
Immunofluorescence: Frozen tissue
Western Blot: 1:500; 1 hr at RT or overnight at 4°C
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the intracellular domain, aa1243-1256 of Nephrin.
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes nephrin. Positively stains podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas. Reacts with PCL (podocyte cell line) and M-1 (cortical collecting duct cells). Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kestila, M., et al., Mol. Cell. 4: 575-582 (1998). 2. Ruotsalainen, V., et al., Proc. Natl. Acad. Sci. USA. 96: 7962-7967 (1999). 3. Holthofer, H., et al., Am. J. Pathol. 155: 1681-1687 (1999). 4. Putaala, H., et al., Hum. Mol. Genet. 10: 1-8 (2001). 5. Huh, W., et al., Nephrol. Dial. Transplant 17: 478-484 (2002). 6. Aaltonen, P., et al., Lab. Invest. 81: 1185-1190 (2001). 7. Bussolati, B., et al., J. Am. Soc. Nephrol. 16: 1936-1947 (2005).