Technical Data
N2028-50A
Nephrin (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)
Description:
Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kD type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa's 1056-1095 are deleted, eliminating the transmembrane region.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~25ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 2-5ug/ml
Immunohistochemistry: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanSheep
Concentration:
As reported
Immunogen:
NS0-derived recombinant human Nephrin (aa23-1029) Accession # O60500
Purity:
Purified by human Nephrin affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human Nephrin. In ELISA and Western Blot, shows ~20% crossreactivity with recombinant mouse Nephrin. Species sequence homology: mouse (84%), canine (89%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.